Brian Lara, the West Indian batting legend scored the most runs on the losing side in a test match, 351 against Sri Lanka in 2001. Andy Flower, a former Zimbabwe batter, is ranked second after scoring 341 runs against South Africa in 2001. Herbert Sutcliffe, a former England batter who scored 303 runs against Australia in 1925, is in third place. The following is a list of batters who scored the most runs on the losing side in a Test match.
| Player | 1st | 2nd | Runs | Team | Opposition | Year |
| Brian Lara | 221 | 130 | 351 | West Indies | v Sri Lanka | 2001 |
| Andy Flower | 142 | 199* | 341 | Zimbabwe | v South Africa | 2001 |
| Herbert Sutcliffe | 176 | 127 | 303 | England | v Australia | 1925 |
| Clyde Walcott | 155 | 110 | 265 | West Indies | v Australia | 1955 |
| Vijay Hazare | 116 | 145 | 261 | India | v Australia | 1948 |
| Herschelle Gibbs | 161 | 98 | 259 | South Africa | v England | 2005 |
| Vinoo Mankad | 72 | 184 | 256 | India | v England | 1952 |
| Virat Kohli | 115 | 141 | 256 | India | v Australia | 2014 |
| Andy Flower | 183* | 70 | 253 | Zimbabwe | v India | 2000 |
| Daryl Mitchell | 190 | 62* | 252 | New Zealand | v England | 2022 |
